crispri backbone pljr962 Search Results


bsmbi digested crispri backbone  (Addgene inc)
95
Addgene inc bsmbi digested crispri backbone
(A) Wild-type strain harbouring the <t>CRISPRi</t> system with different sgRNAs for the promoter region of rho and three positive controls ( DNAβ, MSMEG_0317 , and rho ). (B) Wild-type strain containing the CRISPRi plasmid and a second copy of rho . Strains with pJR962 derivatives expressing sgRNAs were grown to the stationary phase in TSBT in the absence of aTc. The cells were serially diluted 10-fold, and 5 µL of each dilution was spotted onto TSA without (left) or with (right) aTc, which induces expression of the CRISPRi system. Positive controls were designed by .
Bsmbi Digested Crispri Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispri+backbone+pljr962/bio_rxiv__2025__08__28__672908-186-7-12?v=Addgene+inc
Average 95 stars, based on 1 article reviews
bsmbi digested crispri backbone - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier

Image Search Results


(A) Wild-type strain harbouring the CRISPRi system with different sgRNAs for the promoter region of rho and three positive controls ( DNAβ, MSMEG_0317 , and rho ). (B) Wild-type strain containing the CRISPRi plasmid and a second copy of rho . Strains with pJR962 derivatives expressing sgRNAs were grown to the stationary phase in TSBT in the absence of aTc. The cells were serially diluted 10-fold, and 5 µL of each dilution was spotted onto TSA without (left) or with (right) aTc, which induces expression of the CRISPRi system. Positive controls were designed by .

Journal: bioRxiv

Article Title: Phase separation of mycobacterial Rho factor is associated with acid stress

doi: 10.1101/2025.08.28.672908

Figure Lengend Snippet: (A) Wild-type strain harbouring the CRISPRi system with different sgRNAs for the promoter region of rho and three positive controls ( DNAβ, MSMEG_0317 , and rho ). (B) Wild-type strain containing the CRISPRi plasmid and a second copy of rho . Strains with pJR962 derivatives expressing sgRNAs were grown to the stationary phase in TSBT in the absence of aTc. The cells were serially diluted 10-fold, and 5 µL of each dilution was spotted onto TSA without (left) or with (right) aTc, which induces expression of the CRISPRi system. Positive controls were designed by .

Article Snippet: The sgRNA oligos were cloned into the BsmBI-digested CRISPRi backbone (plJR962 - Addgene plasmid #115162).

Techniques: Plasmid Preparation, Expressing

Localization patterns for Msm Rho and its derivatives after 4-hour exposure to TSBT or pH 4 (citrate/phosphate buffer): (A) Wild-type Rho; (B) ΔIDR; (C) Δinitial; (D) Δinsertion. All cultures were supplemented with aTc to repress the native copy of rho through the CRISPRi system. Demographs on the right display the normalized Dendra intensity as heatmaps along the medial axis of single cells (n = 75) which are sorted by length. All analyses were carried out from three independent experiments, and representative images are shown. All scale bars, 2 µm.

Journal: bioRxiv

Article Title: Phase separation of mycobacterial Rho factor is associated with acid stress

doi: 10.1101/2025.08.28.672908

Figure Lengend Snippet: Localization patterns for Msm Rho and its derivatives after 4-hour exposure to TSBT or pH 4 (citrate/phosphate buffer): (A) Wild-type Rho; (B) ΔIDR; (C) Δinitial; (D) Δinsertion. All cultures were supplemented with aTc to repress the native copy of rho through the CRISPRi system. Demographs on the right display the normalized Dendra intensity as heatmaps along the medial axis of single cells (n = 75) which are sorted by length. All analyses were carried out from three independent experiments, and representative images are shown. All scale bars, 2 µm.

Article Snippet: The sgRNA oligos were cloned into the BsmBI-digested CRISPRi backbone (plJR962 - Addgene plasmid #115162).

Techniques: